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PROGRAMME OUTLINE

Inauguration and the workshop will be held at the Department of Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Kelaniya.

Workshop content
  • Setting up a cell culture laboratory
  • Cell Culture Media and consumables
  • Overview of primary and transformed Cell Cultures
  • Cell culture initiation, maintenance, termination and preservation
  • Fluorescence-activated cell sorting (FACS)
  • Cell culture trouble shooting and remedial actions
  • Introduction to basics in molecular biology; Concept of Central Dogma
  • In-vitro amplification of genes: Introduction to PCR
  • DNA extraction and designing PCR protocol for a selected gene
  • Introduction to mutations and genotoxicities
  • In-vitro assessment of Genotoxicity: Cytokinesis Blocked Micronuclei Assay, Chromosomal Aberrations assay
  • Assessment of mutations.
Practical
Practical 1
  • Initiation of primary human peripheral blood lymphocyte (PBLs) cell cultures.
  • Assessment of genotoxicity: Cytokinesis Blocked Micronuclei Assay, Chromosomal Aberrations assay

Practical 2

  • Initiation of human leukemic monocyte (THP1) cells.
  • Assessment of viability: LDH leakage and DNA fragmentation assays.

Practical 3

  • Lymphocyte DNA extraction, purification and quantification
  • Semi-quantitative assessment of selected genes in lymphocytes.

Three practical sessions will be conducted as parallel sessions for three working groups each consists maximum of ten members.